Find your ideal column match based on compound characteristics, pharmacopeia classification, application, or recommended alternative. The usp approach for selecting columns of equivalent. Typically chromatographers must choose hplc columns by comparing the surface area and carbon load data. Hplc columns hplc column selectivity measurements of. It will help you to understand the nature of the product that will help to select the different parameters. The current work describes the application of an automated column selection system using a series of hplc columns varying in hydrophobicity and silanol activity, in combination with.
The current work describes the application of an automated column selection system using a series of hplc columns varying in hydrophobicity and silanol activity, in combination with drylab software to efficiently develop robust, specific and ms compatible hplc methods for the separation of mixtures of drugs andor their metabolites or degradation products. Column selection for size exclusion chromatography. Advances in hplc instrument design, computerized data analysis, and plotting software have added to th. A similarity factor, f s, will be calculated for each of the other columns in the database below. Hplc column selection are you barking up the right tree.
This selection guide makes finding and ordering hplccolumns very easy. Waters uplc, uhplc, and hplc column selection and mobile phase guide. The supelco hplc column selection guide is designed to assist you in choosing the right column for your application based on the features, benefits and specifications of some of our most popular. Our extensive family of products offers a variety of particle sizes and column designs to meet all separation needs, including improved resolution, enhanced sensitivity, faster analysis and consistent performance. Column selection guide low molecular weight organic. Fusion lc method development fusion qbd software quality. You will get the answers by doing only a few clicks in our hplccolumn configurator.
Hplcuhplc column selection chart new approaches for improving hplc and uhplc methodologies within acceptable pharmacopeia guidelines webinar lcmsms analysis of underivitized methylmalonic acid mma and succinic acid in plasma using strataxaw and luna omega 1. The highly configurable graphicaluserinterface gui can be quickly set up to display only the functions necessary for your use. The sample passes through the column with the mobile phase and separates in its. Hplc column comparison screening study for reversed phase columns. First of all consult the literature that is available on the product. Column selection system mistraswitch supplied by erc. There are many column selection guides on the internet. Different types of hplc columns are used in analysis of different pharmaceutical compounds according to their nature and column separation capacity. Column selection for size exclusion chromatography sec hplc. Compare options to find the best product for you, then connect with an expert to discuss your options, or view the product details online to learn more. Hplc column selection guide column parameters internal diameter an important parameter of a hplc column is the internal diameter id as this directly influences the detection sensitivity, selectivity of separation and the quantity of analyte that can be loaded onto the column. To find the columns that are most different from the one selected above, sort the column database below by f s so that the largest f s values are listed first. Uhplc system liquid chromatography hplc shimadzu nexera x2.
Sd flowpath selectors select one of 4 to 16 deadended streams. We help you get the best from your sample preparation and keep you uptodate with the latest software developments. An efficient approach to column selection in hplc method development craig s. With over 40 years as a leader in lc column technology, you can rely on the quality of thermo scientific hplc and uhplc columns. Figures 1 and 2 shows the effects of pore size on peak width and sample load. Application of a column selection system and drylab software. Smatrix is the world leader in qbd experimentation software for the development, validation, and transfer of hplc, uhplc, and sfc methods.
You will get the answers by doing only a few clicks in our hplc column configurator. Reversed phase hplc uhplc chromatography selection tool. With a range of techniques including analytical hplc and uhplc, sample preparation including preparative hplc, we can help you to determine the best hplc column for your needs. Chiralizer services, llc analytical instruments for hplc. Hplc system selection guide thermo fisher scientific us. Gives you the ability to select methods development column kits, choose columns by vendor or by ligand type and quickly find equivalent column chemistries for existing methods, saving you time in your laboratory. A comparison of alltech c18 and c8 columns alltech has developed a method for choosing c18 and c8 columns based on peak capacities and column selectivity of polar and nonpolar compounds.
The great popularity of high performance liquid chromatography hplc systems today empowers chemists to determine the chemical purity of most types of samples. Hplc column comparison screening study for reversed. Select filters to narrow down the list of stationary phases. Relating analyte properties to hplc column selectivity on the road to nirvana the proliferation of hplc column stationary phases over 700 phases, from the various manufacturers, are now available. Column selection for size exclusion chromatography sec standard analysis columns column selection for size exclusion. The infinitylab analytical hplc systems are highly efficient hplc and uhplc systems that feature the latest technology. A comparison of alltech c18 and c8 columns alltech has developed a method for choosing c18 and c8 columns based on peak capacities and.
Tracks the history of hplc column usage for the entire column life cycle from column registration till. Hplc column selection guide chromatography products. Hplc column selection guide column parameters internal diameter an important parameter of a hplc column is the internal diameter id as this directly influences the detection sensitivity, selectivity of. Advantages of using the amplelogic hplc column management software. The selection of the proper hplc column for any given analysis can be an intimidating task for todays analyst. This selection is, neither in terms of manufacturers nor in terms of their products, a complete list, and the accuracy of the data is not. Otherwise, use a column packing with 300a pore size. Jul 28, 2006 the strategy employs an automated column selection system and a series of hplc columns, varying in hydrophobicity and silanol activity, in combination with drylab software to develop chromatographic methods for the separation of mixtures of bupivacaine and its metabolites. What is colimn selection criteria in hplc analysis. Challenges in manual hplc column booking management.
Hilic hplc uhplc columns draw and retain a waterenriched layer onto the surface of the silica which facilitates the interaction of polar compounds with the stationary phase for increased retention. Hplcuhplc column selection chart new approaches for improving hplc and uhplc methodologies within acceptable pharmacopeia guidelines webinar lcmsms analysis of underivitized. You are also able to compare different manufacturers and prices. An efficient approach to column selection in hplc method. Column selection guide low molecular weight organic compounds the chart below is possible to scroll to the left or right. Hplc column management and tracking software amplelogic. Our extensive family of products offers a variety of particle sizes and. Even more choice with updated, free hplc column selection. Column selectivity database select filters to narrow down the list of stationary phases. The shimadzu nexera x2 ultra highperformance liquid chromatograph uhplc is the most advanced chromatographic system available today. Answer a few questions about your current application needs to see which thermo scientific hplc systems would be best suited for your applications. Relating analyte properties to hplc column selectivity on the road to nirvana the proliferation of hplc column stationary phases over 700 phases, from the various manufacturers, are now available makes it impossible to screen the whole selectivity landscape to choose the best phase for your separation.
Hplc column selection hplc column selection to use the column selection guide diagram below, simply follow the path for your analyte and mobile phase. This exdemo column selector offers automated column selection for. The f s factor describes the similarity of two columns. Choosing right column for reverse phase hplc separations 1. By tony taylor how do you choose your hplc columns.
To select multiple values within a box, hold down ctrl while selecting values within the box. Relating analyte properties to hplc column selectivity. Search for hplc column selection guide and look through the options. The majority of chemists will begin with some type of c18 chemistry. Hplc columns hplc column selectivity measurements of more. Gives you the ability to select methods development column kits, choose columns by vendor or by ligand type and quickly find equivalent column chemistries for existing methods, saving you time in your. Reversed phase hplc uhplc chromatography selection tool from. Instead of asking different manufactures for prices or availability, you will find all current hplc columns on one website. Automated column selection and switching systems for hplc. Precision engineered uhplc pumps for pulsefree solvent delivery at up to mpa automated solvent preparation solvent. Hplc and uhplc columns thermo fisher scientific us. This example illustrates a high pressure sd valve or cheminert model c5 used for hplc column selection.
The new version adds 47 new columns to the database. So, choose a stationary phase that is like your molecules. This guide provides one of the most diverse sets of hplc phases available from any company. The 1220 infinity ii lc delivers high quality for an affordable price. Column selection can be easily controlled manually through the front panel or with jasco software. Sge components are designed to be compatible with hplc components from other manufacturers so it is easy to connect sge guards and inline filters to other manufacturers columns as required.
Smatrix is the world leader in qbd experimentation software for the. Columns are the main component in hplc because the column is responsible for the separation of the sample components. Second, they used an analysis of the separation goals in choosing column and particle physical characteristics. The 1260 infinity ii lc is the flexible instrument choice, while the 1260 infinity ii prime lc brings more operational convenience. The usp approach for selecting columns of equivalent selectivity the usp database was developed by the usp working group on column equivalency using the nist srm 870. I guess the answer to this varies widely and ive seen many strategies. I guess the answer to this varies widely and ive seen many strategies being employed including. This technique allows multiple columns to be installed permanently in the system, eliminating instrument downtime and leakage potential resulting from having to change columns repeatedly. All data being displayed was generated by the column manufacturers after evaluation of the chromatographic phase. They will try to develop the separation on that phase by using their knowledge of the analytes and the chromatographic parameters available, such as percent organic. The columns are installed as a part of the loop system, as shown in this drawing. Multiport, highpressure valves coupled to electric.
A small f s indicates that two columns are very similar. Application of a column selection system and drylab. The thermostatic column selection system mistraswitch, is designed for fully automated selection for up to 10 uhplc columns or 10 hplc column. Column selection is the most important part of your hplc method development 1 choose the column according to the your column polarity and ph range of buffer. Jul 29, 2009 there are many column selection guides on the internet. Hplc column selection a comparison of reversedphase columns based on the widely accepted work and data of drs. Overview nexera x2 covers a broad range of uhplc application demands. This exdemo column selector offers automated column selection for analytical and semipreparative hplc.
The supelco hplc column selection guide is designed to assist you in choosing the right column for your application based on the features, benefits and specifications of some of our most popular phases. Chromatographers can use columnselection and columnswitching valves in various ways to improve the development and performance of hplc methods. Waters uplc, uhplc, and hplc column selection and mobile. Relating analyte properties to hplc column selectivity on the. Choosing right column for reverse phase hplc separations.